ArteMice™ Recombinase Zoo

Download PDF

Designation	Strain Name	short description	citation
Deleter / Reporter Mice
Cre deleter	C57BL/6-rosa26 (SA-CrepA)ARTE	general deleter knock-in of NLSSV40T-Cre in ROSA26 locus subsequent deletion of neo selection marker	not publ.
FLPe deleter	C57BL/6-Tg (CAGGS-Flpe)2ARTE	ubiquitous activity random integration of CAGGS promoter - Flpe transgene	not publ.
Arte1 Cre/C31 reporter	B6;B6129S6F1-rosa26 (CAGGS-attB-loxP-PGK- hygroR-attP-loxP- NLSlacZ)ARTE Backcross Generation N5 (14-may-2004)	cre/phiC31 reporter knock-in of lacZ in ROSA26 locus (CAGGSpromoter-attB-loxP- PGKpromoter-hygroR-attP-loxP-NLSlacZ)	not publ.

Inducible Recombinase Mice
rosa (SAcreERT2)	C57BL/6-rosa26(SA- CreERT2)ARTE	inducible general deleter knock-in of high affinity creERT2 in ROSA26 locus successful induction of cre activity after oral tamoxifen administration (5 days, 5 mg oral per day; LD50 of 200 mg), resulting in complete recombination of polBetaFlox reporter in all tissues, except brain (30%) no background activity of uninduced creERT2 also available as ES cell line: ESAR F1 R9 7 (genetic background 129S6B6F1)	Seibler et al., . NAR 2003, 31(4):e12
Mx Cre	B6.Cg-Tg(Mx1-Cre) CGN/ARTE Backcross Generation N15 (14-may-2004)	inducible deleter random integration of NLSSV40T-Cre under control of the interferon-inducible Mx-promoter; mice express Cre in different levels in interferon-reactive tissues; successful induction of cre expression after IP injection of polyInosine-polyCytidine (250 µg each at day 1, 3 and 5), results in deletion of polBetaFlox reporter between 10% (brain) and 100% (liver) low background activity of >10% in spleen, uterus, thymus, heart and lung	Kuehn et al., Science 269: 1427-1429 (1995)

Tissue-specific Recombinase Mice
Cre
CamKII-Cre	B6.Cg-Tg(CamKII-Cre) Backcross Generation N10 (14-may-2004)	forebrain-specific random transgene of NLSSV40T-cre under control of the murine CamKII promoter - efficient recombination in Cortex, Striatum and hippocampus in adult animals	Minichiello et al., Neuron 24: 401-414 (1999)
CD19-Cre	B6.129P2-Cd19tm1 (cre)Cgn/ARTE Backcross Generation N13 (14-may-2004)	B cell-specific knock-in of NLSSV40T-cre into exon 2 of CD19 locus; - 90% deletion of polBetaFlox reporter in splenic B cells, 80% deletion in bone marrow B cell precursors	Rickert et al., NAR 25: 1317-1318 (1997)
MCK-re	B6;Cg-Tg(Mck-Cre) Backcross Generation N5 (14-may-2004)	specific for skeletal and heart muscle random transgene of NLSSV40T-cre under control of the muscle creatin kinase (MCK)promoter - partial deletion, up to 50% of ROSA26 reporter	not publ.
K14-Cre	Stock-Tg (KRT14-Cre)1ARTE	skin- and brain-specific random transgene of NLSSV40T-cre under control of the human keratin 14 promoter - recombination in skin epithelium, brain and blood vessels	unpubl.
NFL-re	Stock-Tg (NFL-Cre)1ARTE	neuron-specific random transgene of NLSSV40T-cre under control of the human neurofilament light chain (NFL) promoter - recombination in individual cells of brain and spinal cord	unpubl.
Flp
CamKII-FLP	C57BL/6-Tg (CamKII-Flpe)	forebrain/hippocampus specific, random transgene of NLSSV40T-FLPe under control of the murine CamKII promoter - efficient recombination in Cortex and hippocampus	unpubl.
Rip-FLP	B6;Cg-Tg(RIP-Flpe) Backcross Generation N7 (14-may-2004)	pancreas beta cells, random transgene of Rat-Insulin_Promoter-FLPe, recombination in pancreas ß-cells	unpubl.
transferrin-FLP	C57BL/6-Tg (TF-Flpe)	brain/liver, random transgene of transferrin-FLPe, recombination in brain and liver, homozygous mice	unpubl.

 
« Back