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Artemis

TECHNOLOGY . ARTEMICE® RNAi
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Artemis has developed a broadly applicable technology for in vivo analysis of any gene in transgenic mice, based upon temporal control of shRNA expression. For the first time it is now possible to inhibit gene expression in mice at a selected point in time and subsequently reactivate the expression of the said gene at selected second point in time. On a broad basis this important novel methodology will enable new insights into gene function in vivo.

Key features of RNAi technology

  • Powerful system for inducible and reversible gene knockdown developed at Artemis1,2
  • Reliable technology - multiple RNAi models have been delivered and analyzed by pharmaceutical industry
  • Time and cost efficient in vivo data within 5 months
  • Gene knockdown in all tissues of the body
  • Data reflect the action of drugs in vivo (70%-95% knockdown of target genes)
  • Suitable technology for high throughput analysis of target genes in vivo


1) Seibler et al.; Reversible gene knockdown in mice using a tight, inducible shRNA expression system. Nucleic Acids Res. 2007 Apr 1;35(7):e54. Epub 2007 Mar 21.
2) Seibler et al.; Single copy shRNA configuration for ubiquitous gene knockdown in mice. Nucleic Acids Res. 2005 Apr 14;33(7):e67

Inducible ArteMice® RNAi mouse models

  • Induced expression of shRNA allows gene knockdown at any time during development upon doxycycline induction (embryo & adult)
  • Inducible RNAi mouse models allow, for the first time, reversible gene knockdown in vivo

    • Key technologies of the RNAi platform

    • Targeted transgenesis by recombinase mediated cassette exchange (RMCE)
    • Single vector based short hairpin (sh) RNA cassettes for constitutive and inducible knockdown
    • Implementation of the RNAi/RMCE technology into the ArteMice® platform allows the fast generation of knockdown models
    • New development of an inbred C57BL/6 cell line with the RMCE-system

    Artemis' inducible system

    • Tightly regulated by a codon optimized tet-repressor
    • Highly inducible upon induction by doxycycline
    • Single inducible cassette for RMCE by placing all necessary regulatory elements on one vector
    • One allele approach by heterozygous insertion into rosa26



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