COMPANY TECHNOLOGY SERVICES PRODUCTS NEWS + EVENTS JOBS
Artemis

SERVICES . ARTEMICE® HUMANIZED™
Impressum Print Page E-Post
This section describes the work flow for the generation of ArteMice® HUMANIZED™ custom made mice.
Terms and conditions for these services are available on request.

  1. Humanized knock-in mice
    Deliverable: 2 breeding pairs of mice heterozygous for the humanized allele
    Time: 42 weeks

    • Locus characterization
    • humanized knock-in Vector construction
    • Transfection into C57BL/6 ES cells
    • ES Cell injection into diploid blastocysts
    • In vivo selection marker deletion (parallel with germline transmission)
    • Breeding to heterozygousity

  2. Humanized and Conditional mice
    Deliverable: 2 breeding pairs of mice heterozygous for the humanized floxed allele
    Time: 44 weeks

    • locus characterization
    • Vector construction allowing humanization and conditional knock-out simultaneously
    • Transfection into C57BL/6 ES cells
    • ES Cell injection into diploid blastocysts
    • In vivo selection marker deletion (parallel with germline transmission)
    • Breeding to heterozygousity

  3. Humanized mice using ES Mice approach
    Deliverable: three male and female homozygous F1 ES mice
    Time: 46 weeks

    • Locus characterization of C57BL/6 and 129 alleles
    • Humanization vector construction for both alleles
    • Consecutive transfection into F1 ES cells to target both alleles
    • In vitro deletion of selection markers
    • Selection of male and female homozygous ES Cells
    • ES Cell injection of male and female homozygous ES Cells

  4. Humanized and Conditional mice using ES Mice approach
    Deliverable: three male and female homozygous F1 ES mice
    Time: 48 weeks

    • Locus characterization of C57BL/6 and 129 alleles
    • Humanization/conditional KO vector construction for both alleles
    • Consecutive transfection into F1 ES Cells containing CreER to target both alleles
    • In vitro deletion of selection markers
    • Selection of male and female homozygous ES Cells
    • ES Cell injection of male and female homozygous ES Cells
Back to top