SERVICES . ARTEMICE^® CONDITIONAL^®

This section describes the work flow for the generation of ArteMice^® CONDITIONAL^® custom made mice. Terms and conditions for these services are available on request.

1. Conditional KO to produce floxed heterozygous mice
   Deliverable: 2 breeding pairs of floxed heterozygous mice
   Time: 40 Weeks
   
   
   • Locus characterization
   • Conditional KO Vector construction
   • Transfection of vector into C57BL/6 ES cells
   • ES Cell injection
   • In vivo selection marker deletion (parallel with germline transmission)
   • Breeding to heterozygosity
     
     Upon request, optional breeding to homozygosity with an ArteMice^® Recombinase Zoo mouse.
     
     Deliverable: 2 breeding pairs of homozygous mice with cre
     Time: 66 Weeks
     
     
2. Conditional KO to produce C57BL/6 homozygous mice containing CreER gene switch
   Deliverable: 2 breeding pairs of homozygous mice with CreER gene switch
   Time: 52 Weeks
   
   
   • Locus characterization
   • Conditional KO Vector construction
     Transfection of vector into C57BL/6 ES cells, containing CreER gene switch
   • ES Cell injection
   • In vivo selection marker deletion (parallel with germline transmission)
   • Breeding to homozygosity
     
     
3. Conditional KO to produce F1 homozygous mice containing CreER gene switch
   Deliverable: three male and female homozygous F1 ES mice
   Time: 44 weeks
   
   
   • Locus characterization of C57BL/6 and 129 alleles
   • Conditional KO Vector construction for both alleles
   • Consecutive transfection into F1 ES Cells containing CreER to target both alleles
   • In vitro deletion of selection markers
   • Selection of male and female homozygous ES Cells
   • ES Cell injection of male and female homozygous ES Cells
     
     
4. Conditional KO to produce F1 heterozygous mice containing CreER gene switch
   Deliverable: five male heterozygous ES mice
   Time: 36 weeks
   
   
   • Locus characterization of C57BL/6 allele
   • Conditional KO Vector construction for one alleles
   • Transfection into F1 ES Cells containing CreER to target one alleles
   • In vitro deletion of selection marker
   • ES Cell injection of male heterozygous ES Cells